WebMay 13, 2024 · LXR and ChREBP interaction was determined by Co-immunoprecipitation (CoIP) and their transactivity was assessed by real-time quantitative polymerase chain … WebChIP-Seq is very powerful but also requires significant bioinformatic expertise. If you are unsure, you can start by looking at a handful of loci and later choose to create a ChIP-Seq library if genome-wide information will be useful. Controls are essential for ChIP. Whether you are comparing the same cell lines or different cell lines and ...
The role of ChREBP in carbohydrate sensing and NAFLD …
WebDec 20, 2024 · To determine this, we reanalyzed two published chromatin immunoprecipitation sequencing (ChIP-seq) datasets; one ChREBP ChIP- seq performed in livers from mice fasted and refed a high-carbohydrate diet (Poungvarinet al, 2015) and one LXR ChIP-seq done in livers from mice treated with the LXR agonist T0901317 … WebThe potential ChoRE for ChREBP binding is highlighted in a red box. C, potential ChoREs in Tug1 promoters of mouse, human, and rat are consensus binding sites for ChREBP. D, ChREBP ChIP-Seq in mouse … glenn beck show stu
ChREBP, a glucose-responsive transcriptional factor, …
http://woundworld.com/ww/k2-7/listing/listing-1-column/item/1704-tcf7l2-in-hepatocytes-regulates-de-novo-lipogenesis-in-diet-induced-non-alcoholic-fatty-liver-disease-in-mice WebChREBP Is a Central Mediator of Glucose-Induced Transcriptional Reprogramming in b Cells (A–D) INS-1E cells were preincubated with 5 mM glucose for 24 hr and subsequently transduced with equal titers of adenovirus expressing constitutive active ChREBP (CA-ChREBP) or GFP as control. The last two decades of research have revealed critical roles of ChREBP in lipid metabolism. ChREBP regulates hepatic lipogenesis via acetyl-CoA produced by the conventional pathway (glycolysis and fructolysis) and a novel pathway (conversion of gut microbiota-derived acetate). It also regulates lipoprotein … See more Excess carbohydrate intake causes hepatic triglyceride accumulation through the activation of carbohydrate response element binding protein (ChREBP) and de novo lipogenesis. Dietary carbohydrate is metabolized to acetyl … See more All the authors (KI, KT, and DY) have made a substantial, direct intellectual contribution to the work, and approved it for publication. See more The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. See more This work was supported by grants from Japan Society for the Promotion of Sciences (JSPS) [KAKENHI Grant 17K00850 and … See more glenn beck show cast