Crispr editing of elite inbred parent lines
WebMar 1, 2024 · Elite line editing by maize HI-Edit a, Model of HI-Edit process in maternal haploid-inducer lines such as maize matl. The star indicates a target site edit in the haploid embryo, which is later ... WebOct 2, 2024 · The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system is the method of choice for plant genome editing projects, as it combines simplicity with efficiency and precision [1, 2].It relies on the nuclease activity of the Cas9 protein, a component of adaptive bacterial defense against bacteriophages or other …
Crispr editing of elite inbred parent lines
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WebMar 4, 2024 · To overcome these problems, we devised haploid induction (HI) editing technology (HI-Edit, Fig. 1a), which enables direct editing of elite inbred lines by a … Full Size Table - One-step genome editing of elite crop germplasm during haploid … Supplementary Figure 1 The V1 Recessive Pale Leaf Phenotype is Used to Identify … Supplementary Figure 3 Sequencing Results From Five Colonies Subcloned … Supplementary Figure 4 Family Tree of The Vlhp Genes in Chinese Spring Wheat, … Supplementary Figure 2 Flow Cytometry Results From Seedling Leaf of Control … Supplementary Figure 9 Ploidy Diagrams of Diploid Wheat and Jswer30a22 Wheat … Supplementary Figure 10 Expression of Cas9 Under Different Promoters - One … Supplementary Figure 7 PCR Colony Sequencing of Gl1 Edited Haploids - … Supplementary Figure 6 Sequencing of The Gw2-2 and Vlhp2 Target Sites in Edited … Supplementary Figure 11 Negative PCR Assays for Putatively Edited Wheat … WebMay 24, 2024 · ZG94 is an elite inbred variety with white flesh and low sugar content for production of edible seeds, and it can also be used as a parent line to produce commercial hybrid seeds. ... Chen Q, Jiang L (2024) CRISPR/Cas9-mediated base-editing system efficiently generates gain-of-function mutations in Arabidopsis. Sci China Life Sci …
WebJul 17, 2024 · Hybrid development requires two main steps: (1) development of inbred lines and, (2) crosses of selected parents to generate hybrid seed. DH technology represents a significant shortcut for the first step, since it allows the obtaining of pure inbred lines in two generations instead of the four to eight generations of self-crossing in ... WebSep 5, 2024 · This clean event with the precise CRISPR-Cas9 edit was used for further validation. Plants hemizygous for the edit were crossed as males to PHFFB, an inbred line containing CMS-C cytoplasm and RF4-Y187, to create an isogenic population segregating 1:1 for the alleles Rf4-Y187/ Rf4-Y187 and Rf4-F187/ Rf4-Y187. This isogenic population …
WebMay 1, 2024 · We created waxy corn hybrids by CRISPR–Cas9 editing of a waxy allele in 12 elite inbred maize lines, a process that was more than a year faster than … WebAug 1, 2024 · Using our CRISPR/Cas9 strategy, a similar allele can be directly created in elite restorer lines and elite inbred cultivars for fast improvement. Elite restorer lines carrying a novel semi-dominant GS2 allele can become valuable resources for breeding of hybrid rice suitable for mechanized seed production, which will help alleviate the high ...
WebCrop breeding aims to generate pure inbred lines with multiple desired traits. Doubled haploid (DH) and genome editing using CRISPR/Cas9 are two powerful game-changing …
WebThus far, this tool has been almost exclusively used for gene knockouts and deletions, with a few examples of gene edits and targeted gene insertions. Here, we demonstrate the … frozen head spWeb(A) The CRISPR-Cas cassette can be transformed into a nonrecalcitrant inbred line for trans editing in an elite recalcitrant inbred line. (B) Pollen carrying a CRISPR-Cas cassette designed to target gene(s) of interest … frozen head state park best trailsWebApr 11, 2024 · To obtain new alleles of the S5 locus, two adjacent targets sites of ORF4 located in exon 1 were selected and constructed into the CRISPR/Cas9 gene editing vectors, and transformed into the inbred HJX74-SSSL 10-06 (Figure 3A). Six T 0 generation plants were amplified by PCR-specific primers, and positive plants were screened. frozen head state park hiking trail mapWebNov 14, 2024 · Backcross breeding is an effective method to transfer one or a few genes controlling a specific trait from one line into a second—usually elite—breeding line. The parent with the desired trait, called the donor parent, provides the desired trait and may not perform as well as an elite variety in other areas. The elite line, called the ... frozen head state park flat fork campgroundWebNov 16, 2024 · In recent years, the multi-parent design named random-open-parent association mapping (ROAM) population was developed, which contained a set of recombinant inbred lines (RILs) families derived from crosses among multiple randomly inter-crossed founder parents. Those founder lines as elite maize inbred lines had … frozen head state park camping reservationsWebFeb 24, 2024 · Humanity first appears as something more than an object of gene editing in the last line of the book: “CRISPR is moving faster than society can keep up. To where is up to all of us.” Yet most ... giant snickers bar near meWebModern maize hybrids often contain biotech and native traits. To-date all biotech traits have been randomly inserted in the genome. Consequently, developing hybrids with multiple traits is expensive, time-consuming, and complex. Here we report using CRISPR-Cas9 to generate a complex trait locus (CTL) to facilitate trait stacking. A CTL consists of multiple … frozen head park