Fitc tag
WebThe Flourotag FITC Conjugation Kit can be used for Immunohistochemisty and Immunocytochemistry using Flow Cytometry. It is also be used for the conjugation of … WebReconstitute one vial of FITC (F7250) in 2 ml of 0.1 M carbonate-bicarbonate buffer and vortex until all FITC has dissolved. Label vial “20:1 FITC”. The solution should be freshly prepared before each conjugation and used within 5 minutes (See Application Note 6). The "20:1 FITC" solution is further used to prepare the "5:1" and "10:1 ...
Fitc tag
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WebNov 20, 2024 · Fluorescein isothiocyanate (FITC) is the derivative of fluorescein with an isothiocyanate reactive group, rendering it reactive towards amine and sulfhydryl groups … WebOct 28, 2014 · 6-FAM and FITC labeled oligo have similar spectral properties. 6-carboxyfluorescein also known as 6-FAM is a single isomer. Fluorescein-5,6-isothiocyanate, is a mix isomers. They are derivatives of the fluorescent dye fluorescein. FITC has a isothiocyanate reactive group (-N=C=S) at one of two hydrogen atoms on the bottom ring …
WebJan 10, 2024 · Tags Alexa Fluor Dyes DAPI Fluorophore Image Restoration and Deconvolution ... Fluorescein isothiocyanate (FITC) is an organic fluorescent dye and probably one of the most commonly used in immunofluorescence and flow cytometry. It has an excitation/emission peak at 495/517 nm and can be coupled to distinct antibodies with … WebApr 13, 2024 · Fluorescence detection is one of the primary analytical methods in biosensing. A fluorescent tag, such as a quantum dot, can serve as a reporter ‘flare’. ... Subsequently, AuNP and FITC were connected by a single aptamer sequence after the peptide cleavage reaction. The distance between the AuNP and FITC was increased. An …
WebOct 13, 2024 · Fluorescein isothiocyanate (generally abbreviated as FITC) is a derivative of fluorescein used in wide-ranging applications mostly because of its low cost. ... 5-FITC) and fluorescein 6-isothiocyanate (6-FITC) (both C 21 H 11 NO 5 S, average mass 389.04 g/mol; Fig. 2). 5-FITC is used as FITC-tags in immunoassays and flow cytometry. They … WebThe antibody is isolated from ascites fluid and conjugated to fluorescein isothiocyanate isomer I (FITC). Monoclonal Anti-c-Myc, FITC conjugate recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C-terminal to the fusion protein. c-Myc is a proto-oncogene localized on long arm of human chromosome 8.
WebProduct Details. Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Streptavidin is conjugated with FITC under optimal conditions. 0.5 mg/ml (concentration relates to the Streptavidin only component of the conjugate) The Streptavidin-FITC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged ...
WebCalculate the quantity of FITC required, by using a molar ratio FITC/ prot of 100. For example, if you want to graft 1mg of protein of 20kDa, the FITC quantity required is 1.9mg. So mix in... greensboro park and rideWebFITC (Fluoresceinisothiocyanate) During peptide synthesis, the peptide can be directly conjugated to the fluorophore at the N-terminus (FITC always passes through the aminocaproic acid (Ahx) linker). For effective N-terminal labeling, a seven-atom aminohexanoyl spacer (NH2-CH2-CH2-CH2-CH2-CH2-COOH) is inserted between the … fmcptd008WebTAG’s headquarters, located on our 5-acre campus in Dulles, Virginia, includes 35,000 sq. ft. of engineering, research and development, systems manufacturing, and Highly … fmc port orangeWebFluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. First described in 1942, FITC is the original … fmc princess anneWebMar 15, 2024 · 产品描述. 本品是由 fitc 标记的兔抗山羊 igg ( h+l ),使用抗原偶联的琼脂糖微珠从兔抗血清内亲和色谱纯化所得。 免疫电泳和 / 或 elisa 法检测显示本品特异性结合完整的山羊 igg 分子,也会与其他山羊免疫球蛋白的轻链结合。 可能会与其他物种免疫球蛋白发生交叉反应,但不会识别非免疫球蛋白 ... fmc propertyWebGenScript THE™ DYKDDDDK Tag Antibody [FITC], mAb, Mouse is FITC conjugated THE™ DYKDDDDK Tag Antibody, mAb, Mouse (A000187) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays. Absorption Wavelength: 492 … fmc port huronWeb6/7/2024 Great HA.11 Epitope Tag Antibody from BioLegend Xu-Qiao Chen High titer and clean. Overall, it is a reliable antibody for western blot. (2) ... 5/23/2016 FITC-Conjugated Monoclonal Mouse Anti-Human CD3 Antibody (for … fmcpnn in digital twins smart healthcare