WebFeb 13, 2024 · The primers are fITS7 and ITS4. PCR program is 25 µl reaction: 1 µl of 1:1 diluted DNA + 0.25 µM primers, 25 cycles (95 °C for 30s, 59 °C for 30 s, 72 °C for 30 s) … Jul 12, 2024 ·

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What is the reason behind use of ITS3 and ITS4 as a

During the last 20 years, analysis of microbial communities based on amplification and sequencing of genetic markers has revolutionized fungal ecology (Horton & Bruns, 2001; Hibbett et al., 2009). High-throughput methods, such as 454-pyrosequencing (Margulies et al., 2005), enable sequencing of … See more We present three new primers, which may be used together with the ITS4 primer to amplify the ITS2 region of fungal templates. The relatively short amplicons lead to high amplification efficiency, reducing the number … See more During PCR amplification of full-length fungal ITS fragments, community composition may be severely distorted because of … See more WebSpore trapping methods for the early detection of new ... WebJul 20, 2024 · The product of the first PCR dilute solution was used as the template for the second PCR under the same conditions as in the first procedure, but the specific primer pair of fITS7 and ITS4 was used for the amplification of the ITS2 regions of fungi. The resulting PCR products were extracted from a 2% agarose gel and further purified using the ... horse trainer mcclanahan